Skip to Content
Merck
All Photos(1)

Key Documents

TK6 (IVGT)

13051501, human spleen, Small round cells in suspension

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

Pricing and availability is not currently available.

product name

TK6 (IVGT), from human spleen, 13051501

biological source

human spleen

description

Human lymphoblast, thymidine kinase heterozygote, mutagenesis assay, diploid human

growth mode

Suspension

morphology

Small round cells in suspension

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Description

JCRBNo 1435, lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.

Culture Medium

RPMI 1640 (R0883) + 10% Horse Serum + 2mM L-Glutamine (G7513) + 1.8 mMolar Sodium Pyruvate(NaP) (S8636). Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)

Subculture Routine

Dilute cell in fresh medium at 0.1- 1.5 x 105 cells per ml.

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: TK6 (IVGT) (ECACC 13051501)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service