A9853
Anti-Androgen Receptor antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Androgen Receptor Antibody, Androgen Receptor Antibody - Anti-Androgen Receptor antibody produced in rabbit, Anti-AR
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 110 kDa (additional band may be observed at 45 kDa)
species reactivity
rat, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections
immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg)
microarray: suitable
western blot: 1-2 μg/mL using whole extract of rat brain
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Androgens are the male sex hormones that exert biological and transcriptional effects on binding to nuclear androgen receptor (AR). Androgens in humans include androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone. Androgen receptor is a ligand inducible nuclear receptor, expressed on brain, bone, skeletal muscles, genital organs, hair follicles and cardiovascular tissue. On binding androgen, the AR is internalized and modulates the transcriptional of target genes. Additionally, AR also exerts extra-nuclear actions wherein it rapidly induces signalling cascades of MAPK and protein kinase C. The regulation by AR results in irreversible sexual differentiation of sex organs during embryogenesis and development of sex-specific characteristics during puberty
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.
Specificity
The antibody cross-reacts with androgen receptor from rat.
Immunogen
evolutionally conserved peptide corresponding to amino acids 1-21 of the human androgen receptor. This sequence is not found in estrogen, progesterone, or glucocorticoid receptors.
Application
Anti-androgen receptor antibody may be used for immunoblotting using whole rat brain extract at a recommended concentration of 1-2 μg/ml. For immunoprecipitaion from 250 μg of human MCF7 cell lysate, a concentration of 5-10 μg may be used. Immunohistochemistry may be performed on paraffin-embedded human prostate tissue sections at a working antibody concentration of 20-40 μg/ml.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit anti-androgen receptor (C19) was used in a EMSA (electrophoretic mobility shift assay) which produced a supershift in the molecular weight of a particular DNA sequence indicating binding of the androgen receptor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Laura H Porter et al.
The Prostate, 79(11), 1326-1337 (2019-06-19)
Serially transplantable patient-derived xenografts (PDXs) are invaluable preclinical models for studying tumor biology and evaluating therapeutic agents. As these models are challenging to establish from prostate cancer specimens, the ability to preserve them through cryopreservation has several advantages for ongoing
D Orlic et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(18), 10344-10349 (2001-08-16)
Attempts to repair myocardial infarcts by transplanting cardiomyocytes or skeletal myoblasts have failed to reconstitute healthy myocardium and coronary vessels integrated structurally and functionally with the remaining viable portion of the ventricular wall. The recently discovered growth and transdifferentiation potential
Qing Xie et al.
The Journal of biological chemistry, 292(33), 13521-13530 (2017-07-07)
NK3 homeobox 1 (Nkx3.1), a transcription factor expressed in the prostate epithelium, is crucial for maintaining prostate cell fate and suppressing tumor initiation. Nkx3.1 is ubiquitously expressed in luminal cells of hormonally intact prostate but, upon androgen deprivation, exclusively labels
Urmila Santanam et al.
Genes & development, 30(4), 399-407 (2016-02-18)
Understanding new therapeutic paradigms for both castrate-sensitive and more aggressive castrate-resistant prostate cancer is essential to improve clinical outcomes. As a critically important cellular process, autophagy promotes stress tolerance by recycling intracellular components to sustain metabolism important for tumor survival.
N Yadav et al.
Minerva urologica e nefrologica = The Italian journal of urology and nephrology, 64(1), 35-49 (2012-03-10)
The embryonic development, growth and maturation of the prostate relies on androgens, the male sex steroids, acting through their cognate receptor, the androgen receptor (AR). This dependence on androgens continues in adult life, where AR signaling remains necessary for the
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