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A6306

Sigma-Aldrich

Agarase from Pseudomonas atlantica

lyophilized powder, ≥5,000 units/mg protein (Lowry)

Synonym(s):

β-Agarase, Agarose 4-glycanohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42040102
NACRES:
NA.26

biological source

bacterial (Pseudomonas atlantica)

Quality Level

form

lyophilized powder

specific activity

≥5,000 units/mg protein (Lowry)

storage temp.

2-8°C

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Unit Definition

One unit will produce 1.0 μg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.

Physical form

Product is formulated with phosphate buffered salts, bovine serum albumin and Agarase. Total protein content ranges between 10 – 30% w/w.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Seungwoo Lee et al.
Journal of microbiology and biotechnology, 21(11), 1116-1122 (2011-12-01)
In this study, site-directed mutagenesis was performed on the β-agarase AgaA gene from Zobellia galactanivorans to improve its catalytic activity and thermostability. The activities of three mutant enzymes, S63K, C253I, and S63K-C253I, were 126% (1,757.78 U/mg), 2.4% (33.47 U/mg), and
Effects of temperature, light level, photoperiod, and ammonium concentration on Pyropia leucosticta (Bangiales, Rhodophyta) from the Northwest Atlantic
Lindsay Gree
Journal of Applied Phycology (2015)
?-Agarases I and II from Pseudomonas atlantica
Lora M
FEBS Journal (1983)
Young Joon Rhee et al.
Journal of microbiology and biotechnology, 20(10), 1378-1385 (2010-10-30)
A novel agarolytic bacterium KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of gram-staining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified
Uyangaa Temuujin et al.
Applied microbiology and biotechnology, 92(4), 749-759 (2011-06-10)
The DagA product of Streptomyces coelicolor is an agarase with a primary translation product (35 kDa) of 309 amino acids, including a 30-amino acid signal peptide. Although dagA expression in Streptomyces lividans under the control of its own set of

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