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A2350

Sigma-Aldrich

AlumaSeal® II film

non-sterile

Synonym(s):

Sealing film

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About This Item

UNSPSC Code:
41122410
NACRES:
NB.15

material

aluminum film

sterility

non-sterile

feature

piercable

manufacturer/tradename

Excel Scientific AF-100

packaging

pkg of one zippered bag of 100

parameter

-80 to +120 °C temp. range

technique(s)

PCR: suitable

L

146.1 mm

W × L

8.26 cm × 14.61 cm

W

82.6 mm

foreign activity

RNase/ DNase, certified free

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General description

A soft aluminum foil film designed for the sealing of PCR plates minimizing evaporation and vapor loss during thermal cycling. The film pierces very easily for direct sample recovery with a multichannel pipettor or robotic metal probes without risk of aerosols, cross-contamination or significant gumming of adhesive. An extremely strong, medical-grade adhesive provides a uniform, complete seal which can withstand PCR thermal cycling. Recommended for temperatures from -80 °C to 120 °C, measures 82.6 mm x 146.1 mm and offers sufficient sealing area for all PCR plates. A 36 μm non-permeable soft aluminum foil film eliminates the need for heat-sealing devices or mats during thermal cycling. The proprietary adhesive can withstand harsh chemicals, solvents and reagents. Two end tabs for easy and accurate positioning and labeling.

Legal Information

AlumaSeal is a registered trademark of Excel Scientific, Inc.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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