F6377

Fluorescein sodium salt

used as fluorescent tracer

• Synonym(s): Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
• Empirical Formula (Hill Notation): C₂₀H₁₀Na₂O₅
• CAS Number: 518-47-8
• Molecular Weight: 376.27
• Colour Index Number: 45350
• Beilstein: 3833041
• EC Number: 208-253-0
• MDL number: MFCD00167039
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894907
• NACRES: NA.47

Properties

• Quality Level: 200
• form: powder
• color: orange to brown
• pH: 8.3 (20 °C, 10 g/L)
• mp: >300 °C
• solubility: H₂O: 1 mg/mL
• density: 0.78 g/cm³ at 29.6 °C (85.3 °F at 699.7 hPa)
• fluorescence: λ_ex 460 nm; λ_em 515 nm(lit.)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
• InChI key: RGPLGPBQJOQFJS-UHFFFAOYSA-L

Description

General description

Fluorescein is a xanthene dye and is a common labeling agent in biology.

Application

Fluorescein sodium salt has been used as a solute to study microvessel permeability. It has been used as a test molecule to study blood barrier penetration.

Water soluble fluorescent dye used in permeability and vascular perfusion studies due to its small molecular radius. Sodium fluorescein is used as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models. The dye was used as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes. Sodium fluorescein was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): 423.7 °F - Pensky-Martens closed cup
• Flash Point(C): 217.6 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves