86348

TCBS Agar

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): Vibrio Selective Agar, Thiosulfate Citrate Bile Sucrose Agar
• UNSPSC Code: 41171606
• NACRES: NA.74

Properties

• Agency: according to ISO 21872-1:2017; according to ISO 21872-2:2007
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 14 g/L ; bromothymol blue, 0.04 g/L ; ferric citrate, 1 g/L ; mixed peptone, 10 g/L ; ox-bile, 5 g/L ; sodium chloride, 10 g/L ; sodium cholate, 3 g/L ; sodium citrate, 10 g/L ; sodium thiosulfate, 10 g/L ; sucrose, 20 g/L ; thymol blue, 0.04 g/L ; yeast extract, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 8.6±0.2 (25 °C)
• application(s): environmental; food and beverages; microbiology
• suitability: selective and differential for Vibrio spp.

Description

General description

Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.

Application

Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.

Preparation Note

Dissolve 88 g in 1 litre distilled water and bring to the boil. Pour plates. Do NOT autoclave.

Other Notes

Incidence of Vibrio cholerae from estuaries of the United States West Coast; Survival and multiplication of Vibrio cholerae serotype Ogawa in reconstituted infant milk

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves