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4B200

Sigma-Aldrich

Sepharose™ 4B

45-165 μm bead diameter

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About This Item

CAS Number:
9012-36-6
MDL number:
MFCD00081294
UNSPSC Code:
12352200
PubChem Substance ID:
24873317
NACRES:
NA.21

biological source

algae (marine)

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose™ 4B has been used:
  • to conjugate scorpion venom to be used in affinity chromatography
  • for clearing of protein extracts prior to immunoprecipitation
  • for gel purification and desalting of phospholipid vesicles
  • for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
MKCP7255
MKCP4639
MKCP4380
MKCP0262
MKCP0261

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Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Adamala K, et al.
Nature Protocols, 10(6), 927-927 (2015)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Hosseini Z, et al.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
Saddam M Muthana et al.
PloS one, 10(3), e0119298-e0119298 (2015-03-26)
Anti-glycan antibodies are an abundant subpopulation of serum antibodies with critical functions in many immune processes. Changes in the levels of these antibodies can occur with the onset of disease, exposure to pathogens, or vaccination. As a result, there has
Paul Kaufmann et al.
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
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