SCM018
Embryoid Body (EB) Formation Medium
The Embryoid Body Formation Medium (EB Media) has been optimized & qualified to support the formation of embryoid body.
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
Quality Level
form
liquid
technique(s)
cell differentiation: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
shipped in
dry ice
General description
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.
The Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.
Physical form
Embryoid Body (EB) Formation Medium is a proprietary formulation that contains fetal bovine serum.
Storage and Stability
Stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Articles
rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
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