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MABS453

Sigma-Aldrich

Anti-Axin2, clone cond4-1D5 Antibody

clone cond4-1D5, from rat

Synonym(s):

Axin-2, Axin-like protein, Axil, Conductin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

cond4-1D5, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_004646

UniProt accession no.

Q9Y2T1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AXIN2(8313)

General description

Axin-2, or Axis inhibition protein 2, is an inhibitor of the Wnt signaling pathway that down-regulates beta-catenin and APC through phosphorylation. Axin-2 abnormalities are linked to defective mismatch repair and have been found to be involved in colorectal cancer (CRC) and oligodontia-colorectal cancer syndrome (ODCRCS). Axin-2 can be found in brain and lymphoblast tissues.

Specificity

This monoclonal antibody recognizes both endogeneous and overexpressed Axin2 and does not cross-react with Axin1

Immunogen

Linear peptide corresponding to Human Axin 2.

Application

Immunocytochemistry Analysis: 20 μg/mL of this antibody from a representative lot detected Axin2 in HeLa, HEK293 and NIH-3T3 cells.
Western Blotting Analysis: A representative lot of this antibody detected Axin2 in SW 480 cell lines
Research Category
Signaling

Apoptosis & Cancer
Research Sub Category
General Post-translation Modification
This Anti-Axin2, clone cond4-1D5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Axin2.

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 1 μg/mL of this antibody detected Axin2 in 10 µg of HCT116 cell lysate.

Target description

~ 95 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ute Schaal et al.
International journal of colorectal disease, 28(11), 1469-1478 (2013-05-25)
Aberrant activation of the Wnt/β-catenin pathway plays a major role in the development of colorectal carcinoma (CRC). Axin 2 is a key protein of this pathway and is upregulated in CRC. Here, we investigated RNA- and protein expression of axin

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