MABF3034

Anti-NKG2D/CD314 Antibody, clone A10

• Synonym(s): B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13B
• UNSPSC Code: 12352203

Properties

• biological source: hamster
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: A10, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable; inhibition assay: suitable
• isotype: IgGκ
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_001076791
• UniProt accession no.: O54709
• Gene Information: mouse ... Tnfsf13b(27007)

Description

Specificity

Clone A10 is an Armenian Hamster monoclonal antibody that detects NKG2-D type II integral membrane protein (NKG2D/CD314).

Immunogen

Recombinant fragment containing the extracellular domain of mouse NKG2D/CD314.

Application

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected NKG2D/CD314 in one million C57BL/6 mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected NKG2D/CD314 in Flow Cytometry applications (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).

Agonist Function: A representative lot stimulated the production of GM-CSF by KY-1 NK clones and C57BL/6 LAK cells and also stimulated NK cells to kill Daudi targets. (Ho, E.L., et al. (2002). J Immunol. 169(7):3667-75).

Agonist Functon: A representative lot increased Vav phosphorylation in freshly isolated splenic C57BL/6 NK cells. (Ghasemi, R., et al. (2016). Nat Commun. 7:12878).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).

Physical form

Purified Armenian Hamster monoclonal antibody IgG in PBS without azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable