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Key Documents

MABF161A

Sigma-Aldrich

Anti-CD45 (human), violetFluor® 450, clone HI30 Antibody

clone HI30, from mouse, violetFluor® 450

Synonym(s):

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

violetFluor® 450

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

HI30, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

P06800

target post-translational modification

unmodified

Gene Information

human ... PTPRC(5788)

Related Categories

General description

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Application

This mouse monoclonal Anti-CD45 Antibody (human), violetFluor® 450, clone HI30, Cat. No. MABF1611 is validated for use in Flow Cytometry, for the detection of CD45.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 1 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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