MAB3060
Anti-Paxillin Antibody, clone 349
clone 349, Chemicon®, from mouse
Synonym(s):
Anti-AW108311, Anti-AW123232, Anti-Pax
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
349, monoclonal
species reactivity
rat, human, mouse, canine, chicken
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PXN(5829)
rat ... Pxn(360820)
Specificity
Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.
Immunogen
Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.
Application
Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Western blot: 1:1,000-1:10,000
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-581
Physical form
Format: Purified
Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analysis Note
Control
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals.
Stylianou, P; Skourides, PA
Mechanisms of Development null
Contact between human bone marrow stromal cells and B lymphocytes enhances very late antigen-4/vascular cell adhesion molecule-1-independent tyrosine phosphorylation of focal adhesion kinase, paxillin, and ERK2 in stromal cells.
L J Jarvis, J E Maguire, T W LeBien
Blood null
F N Leeuwen et al.
The Journal of cell biology, 139(3), 797-807 (1997-11-14)
The invasion-inducing T-lymphoma invasion and metastasis 1 (Tiam1) protein functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac1. Differentiation-dependent expression of Tiam1 in the developing brain suggests a role for this GEF and its effector Rac1
Matrix nonenzymatic glycosylation leads to altered cellular phenotype and intracellular tyrosine phosphorylation.
Hasegawa, G, et al.
The Journal of Biological Chemistry, 270, 3278-3283 (1995)
Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton.
R M Ezzell, W H Goldmann, N Wang, N Parashurama, N Parasharama, D E Ingber, R M Ezzell et al.
Experimental Cell Research null
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