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Key Documents

MAB2040

Sigma-Aldrich

Anti-Heparin/Heparan Sulfate Antibody, clone T320.11

clone T320.11, Chemicon®, from mouse

Synonym(s):

Anti-Heparan Sulfate, Clone T320.11 Anti-Heparin, Heparan Sulfate Detection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T320.11, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HSPG2(3339)

Specificity

Reacts with heparin/heparan sulfate. Low affinity cross-reactivity with DNA. It is reported that clone also cross reacts with dextran sulfate (Mahmood, 2002).

Application

Detect Heparin/Heparan Sulfate using this Anti-Heparin/Heparan Sulfate Antibody, clone T320.11 validated for use in ELISA, RIA & WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western blotting (recognizes heparan sulfate proteoglycan) 1:1000

EIA/RIA: Suggested concentration 1-10 μg/mL.

Optimal working dilutions must be determined by end user.

Target description

64 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Widely expressed

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Obligatory role for endothelial heparan sulphate proteoglycans and caveolae internalization in catestatin-dependent eNOS activation.
Fornero, S; Bassino, E; Ramella, R; Gallina, C; Mahata, SK; Tota, B; Levi, R; Alloatti, G; Gallo, MP
BioMed Research International null
The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine residue.
Vanpouille, C; Deligny, A; Delehedde, M; Denys, A; Melchior, A; Lienard, X; Lyon et al.
The Journal of Biological Chemistry null
Julia Hosp et al.
PloS one, 7(7), e40172-e40172 (2012-07-14)
Extracellular matrices regulate biological processes at the level of cells, tissues, and in some cases, entire multicellular organisms. The subphylum Urochordata exemplifies the latter case, where animals are partially or completely enclosed in "houses" or "tunics". Despite this common strategy
Extracellular superoxide dismutase protects against matrix degradation of heparan sulfate in the lung.
Kliment, CR; Tobolewski, JM; Manni, ML; Tan, RJ; Enghild, J; Oury, TD
Antioxidants & Redox Signaling null
Syndecan-1/CD147 association is essential for cyclophilin B-induced activation of p44/42 mitogen-activated protein kinases and promotion of cell adhesion and chemotaxis.
Pakula, R; Melchior, A; Denys, A; Vanpouille, C; Mazurier, J; Allain, F
Glycobiology null

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