IHCR2025-6
IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3
clone AE1/AE3, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AE1/AE3, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
IHC Select
technique(s)
immunohistochemistry: suitable (paraffin)
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRT1(3848)
General description
Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (Moll, R., 1982). These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50′, 48, 46, 45, and 40 kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52 kD. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (Sun, T.T., 1984; Cooper, D., 1985; Sun, T. T., 1985 ). The 56.5/65-67 kD pair is present in keratinized (differentiated) epidermis. The 55/64 kD pair is characteristic of normal (corneal-type) epithelial differentiation (Moll, R., 1982; Sun, T.T, 1984). The 51/59 kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (Moll, R., 1982; Cooper, D., 1985). The 51/58 kD pair is a keratinocyte marker; this pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (Moll, R., 1982; Sun, T.T., 1984). The 48/56 kD pair is characteristic of hyper-proliferative (de-differentiated) keratinocytes (Moll, R., 1982; Weiss, R.A., 1984). The 45/52 kD pair and to a lesser extent, the 46/54 kD pair are characteristic of simple epithelia (Moll, R., 1982). The 40 kD keratin is present in most epithelia except adult epidermis (Moll, R., 1982).
Specificity
Anti-Keratin AE1 recognizes the 56.5, 50, 50′, 48, and 40 kD keratins of the acidic subfamily. Anti-keratin AE3 recognizes all members of the basic subfamily. AE1/AE3 reacts with both acidic and basic keratins. Staining is localized to the cytoplasm. AE1/AE3 specifically binds to antigens located in the cytoplasm of normal epithelia. Reacts with cells of epithelial origin including simple and stratified epithelia and epidermis.
Immunogen
Human epidermal keratin
Application
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.
Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.
Incubation: 10 minutes with IHC Select Detection Kits.
Cytokeratin AE1/AE3 has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2025-6.
Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.
Incubation: 10 minutes with IHC Select Detection Kits.
Cytokeratin AE1/AE3 has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2025-6.
IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Physical form
Format: Purified
Purified monoclonal mouse antibody supplied in liquid format diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
Storage and Stability
Maintain at 2-8°C . Refer to vial for expiration dating.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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