HRNKLMAG-51K-01

MILLIPLEX^® Human RANKL Magnetic Bead- Single Plex - Metabolism Assay

This MILLIPLEX MAP Human RANKL Magnetic Bead- Single Plex, HRNKLMAG-51K-01 is a Metabolism single plex assay. The analyte available for this kit is RANKL.

• Synonym(s): Human RANKL Detection, Human RANKL Magnetic Beads, RANKL Magnetic Bead Kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 92%; sensitivity: 5.0 pg/mL; standard curve range: 4.88-20,000 pg/mL
• technique(s): multiplexing: suitable
• UniProt accession no.: O14788; O35235
• compatibility: configured for Single Plex
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Bone metabolism is the dynamic process of ongoing bone deposition and re-absorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve appropriate rates of growth and differentiation. The protein Receptor Activator for Nuclear Factor B Ligand (RANKL) activates osteoclasts and has been implicated in degenerative bone diseases such as rheumatoid arthritis and osteomyelitis.

MILLIPLEX^® Human RANKL Single Plex Kit is used for the quantification of RANKL in human serum, plasma and cell / tissue culture supernatant samples. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Application

• Analyte: RANKL
• Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants and lysates
• Recommended Sample Dilution: 25 uL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution with an appropriate control medium.
• Assay Run Time: Overnight (16-18 hr) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Bone

Packaging

Everything you need in a single kit.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H319,H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects