HNPMAG-35K

MILLIPLEX^® Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay

The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.

• Synonym(s): Human Neuroscience Protein Multiplex Assay, Luminex^® Human Neuroscience Panel, Millipore Human Neuroscience Immunoassay
• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 98-118%; (Spike Recovery); sensitivity: 11-479 pg/mL; (MinDC+2SD); standard curve range: 27-20,000 pg/mL; (α-MSH); standard curve range: 41-30,000 pg/mL; (Neurotensin); standard curve range: 55-40,000 pg/mL; (Oxytocin); standard curve range: 69-50,000 pg/mL; (β-Endorphine); standard curve range: 7-5,000 pg/mL; (Substance P)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The central nervous system is a complex environment consisting of billions of neurons and glial support cells. These neurons use chemical signals to communicate information. Neuropeptides are neuronal signaling molecules secreted by the central and peripheral nervous systems, influencing a broad spectrum of biological functions including the regulation of metabolism, reproduction, immunity, and behavior. Understanding the role of neuropeptides in the pathogenesis of devastating neuronal diseases and behavioral disorders is fundamental. While it is understood that accurate measurement of neuropeptides is important, conventional methods, including RIAs and ELISAs, are not able to simultaneously measure multiple neuropeptides with small sample volume. MILLIPLEX^® Human Neuropeptide Bead Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples: α-Melanocyte Stimulating Hormone (α-MSH), β-Endorphin, Neurotensin, Oxytocin, and Substance P.

The MILLIPLEX^® Human Neuropeptide Bead Panel is to be used for the simultaneous quantification of the following 5 analytes in any combination: α-MSH, β-Endorphin, Neurotensin, Oxytocin, and Substance P. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate, culture supernatant samples, and cerebrospinal fluid (CSF). Serum or plasma samples may also be used and will need to be extracted. An important note about this conversion panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.

Panel Type: Neuroscience

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P
• Note that this is a competitive assay.
• Recommended Sample type: serum, plasma, tissue culture supernatant, or CSF
• Recommended Sample dilution: extracted serum or plasma or neat CSF or culture supernatant
• Assay Run Time: Overnight
• Research Category: Neuroscience

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Human Neuropeptide Standard, 1 vial, lyophilized

Human Neuropeptide Quality Control 1, 1 vial, lyophilized

Human Neuropeptide Quality Control 2, 1 vial, lyophilized

Assay Buffer, 2 bottles, 30 mL ea.

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Human Neuropeptide Primary Antibodies, 1 bottle, 3.5 mL

Human Neuropeptide Detection Antibodies, 1 bottle, 5.5 mL

Human Neuropeptide Matrix Solution, 1 bottle, 1.5 mL

Human Neuropeptide Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.