AB4340
Anti-Zscan4 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Zinc finger and SCAN domain containing protein 4C
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
Zinc finger and SCAN domain containing protein 4, also called Zscan4, is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency. Zscan4 binds and regulates telomere elongation in order to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells.
Immunogen
KLH-conjugated linear peptide corresponding to mouse Zscan4.
Application
Anti-Zscan4 Antibody is a highly specific rabbit polyclonal antibody, that targets Zinc Finger Protein & has been tested in western blotting, Immunofluorescence & IP.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Zscan4 in mouse iPS and R1 Embyonic Stem cells. Data courtesy of the Melanie Welham lab at The University of Bath, UK
Immunoprecipitation Analysis: A 1:4,000 dilution from a representative lot detected Zscan4 in cytoplasmic and nuclear extracts in IP experiments conducted by the Melanie Welham lab at The University of Bath, UK. (Data not shown)
Immunoprecipitation Analysis: A 1:4,000 dilution from a representative lot detected Zscan4 in cytoplasmic and nuclear extracts in IP experiments conducted by the Melanie Welham lab at The University of Bath, UK. (Data not shown)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blotting in mouse Embryonic Stem cell lysates.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Zscan4 in 10 µg of Embryonic Stem cell lysates.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Zscan4 in 10 µg of Embryonic Stem cell lysates.
Target description
~65 kDa observed. The calculated molecular weight of this protein is 57 kDa An uncharacterized band may be observed at ~50 kDa in some cell lysates.ZSCAN4 bands may be observed at both ~72 kDa and ~40 kDa when preparing cell lysates with SDS buffer. Additionally, a ZSCAN4 doublet may be observed at ~72 kDa when using cytosolic or nuclear extracts.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Embryonic Stem Cell lysates
Embryonic Stem Cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lei Yang et al.
EMBO reports, 21(9), e50054-e50054 (2020-07-28)
Cloned animals generated by somatic cell nuclear transfer (SCNT) have been reported for many years; however, SCNT is extremely inefficient, and zygotic genome activation (ZGA) is required for SCNT-mediated somatic cell reprogramming. To identify candidate factors that facilitate ZGA in
Zscan4 is regulated by PI3-kinase and DNA-damaging agents and directly interacts with the transcriptional repressors LSD1 and CtBP2 in mouse embryonic stem cells.
Storm, MP; Kumpfmueller, B; Bone, HK; Buchholz, M; Sanchez Ripoll, Y; Chaudhuri, JB; Niwa et al.
Testing null
Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay.
Xiaoying Ye et al.
Frontiers in cell and developmental biology, 9, 675411-675411 (2021-06-15)
It has been well established that leukemia inhibitory factor (LIF) is essential for maintaining naïve pluripotency of embryonic stem cells (ESCs). Oncostatin M (OSM) is a member of the IL-6 family of cytokines which share gp130 as a receptor subunit
Role of Tet proteins in enhancer activity and telomere elongation.
Lu, F; Liu, Y; Jiang, L; Yamaguchi, S; Zhang, Y
Genes & Development null
Deepika Puri et al.
EMBO reports, 22(12), e53180-e53180 (2021-10-05)
Repeat element transcription plays a vital role in early embryonic development. The expression of repeats such as MERVL characterises mouse embryos at the 2-cell stage and defines a 2-cell-like cell (2CLC) population in a mouse embryonic stem cell culture. Repeat
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