AB1511
Anti-Glutamate Decarboxylase 65 & 67 Antibody
Chemicon®, from rabbit
Synonym(s):
65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2, glutamate decarboxylase 2 (pancreatic islets and brain,65kD), glutamate decarboxylase 2 (pancreatic islets and br
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, feline, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GAD2(2572)
General description
Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
Specificity
Glutamate Decarboxylase (GAD65 and GAD67).
Western blot reveals a doublet at approximately 65/68 kDa.
Immunohistochemical staining can be abolished by preincubation with 1-10 μg peptide (Catalog Number AG252) per mL of diluted antibody.
Western blot reveals a doublet at approximately 65/68 kDa.
Immunohistochemical staining can be abolished by preincubation with 1-10 μg peptide (Catalog Number AG252) per mL of diluted antibody.
Immunogen
A synthetic peptide with the amino acid sequence [C]DFLIEEIERLGQDL from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys] + 572-585) (1).
Application
Immunohistochemistry:
1:200-1:5,000 dilution of a previous lot was used in immunohistochemistry (overnight PAP or ABC). Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Also reactive on paraffin embedded tissue. Protease digestion is not required.
Optimal working dilutions must be determined by the end user.
1:200-1:5,000 dilution of a previous lot was used in immunohistochemistry (overnight PAP or ABC). Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Also reactive on paraffin embedded tissue. Protease digestion is not required.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
This Anti-Glutamate Decarboxylase 65 & 67 Antibody is validated for use in IH, WB for the detection of Glutamate Decarboxylase 65 & 67.
Quality
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected GAD 65&67 on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected GAD 65&67 on 10 μg of mouse brain lysates.
Target description
65/68 kDa
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal in buffer containing PBS-BSA containing 0.1% sodium azide.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Whole rat brain lysate, mouse brain cell extract.
Whole rat brain lysate, mouse brain cell extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ventrally located commissural neurons express the GABAergic phenotype in developing rat spinal cord.
P E Phelps et al.
The Journal of comparative neurology, 409(2), 285-298 (1999-06-24)
Early-forming commissural neurons are studied intensively as a model of axonal outgrowth and pathfinding, yet the neurotransmitter phenotype of the majority of these neurons is not known. The present study has determined that a substantial number of commissural neurons express
Hiroshi Katsuki et al.
Neuroreport, 16(11), 1157-1161 (2005-07-14)
Selective degeneration of hypothalamic orexin neurons, a hallmark of pathology in narcolepsy patients, is in part reproduced in hypothalamic slice cultures by application of an endogenous excitotoxin quinolinic acid. Depolarized membrane potential may be responsible for the vulnerability of orexin
A El Idrissi et al.
Neuroscience, 156(3), 693-699 (2008-08-30)
Taurine, 2-aminoethanesulfonic acid, is one of the most abundant free amino acids especially in excitable tissues, with wide physiological actions. We have previously reported that in mice, supplementation of the drinking water with taurine induces alterations in the inhibitory GABAergic
Manganese ethylene-bis-dithiocarbamate and selective dopaminergic neurodegeneration in rat: a link through mitochondrial dysfunction.
Zhang, Jing, et al.
Journal of Neurochemistry, 84, 336-346 (2003)
Hearing loss alters the subcellular distribution of presynaptic GAD and postsynaptic GABAA receptors in the auditory cortex.
Sarro, EC; Kotak, VC; Sanes, DH; Aoki, C
Cerebral Cortex (1991)
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