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70073-M

Sigma-Aldrich

pET-30 Xa/LIC Vector Kit - Novagen®

Novagen′s pET-30 Xa/LIC vector is designed for cloning and high-level expression of target proteins fused with the His-Tag and S-Tag coding sequences that are cleavable with Factor Xa protease.

Synonym(s):

Xa/LIC Cloning Kits, pET-30 Xa/LIC vector

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About This Item

UNSPSC Code:
41106610
NACRES:
NA.54

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−70°C

General description

The pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S•Tag coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.

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Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

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