Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RAB13(5872)
General description
Rab proteins are members of the Ras superfamily of small G-Proteins. At least 60 isoforms have been identified. These GTPases are primarily involved in vesicle trafficking and it is believed that a different Rab protein may be needed for each step of the vesicular transport. It is thought Rab4 and Rab11 are involved in internalization, sorting, and transport to lysosomes or recycling, of several membrane proteins, including GPCRs, GLUT-4, Aquaporin-2, or the H+/K+ ATPase.
Specificity
Recognizes Rab13
Immunogen
Sythnetic peptide of amno acids 129-148 of rat Rab13
Application
Detect Rab13 using this Anti-Rab13 Antibody validated for use in WB, IP & IF.
Immunofluorescence Staining:
Independent laboratory demonstrated this antibody worked in Immunofluorescence studies at a 1:100-1:500 dilution.
Immunoprecipitation (IP):
Independent laboratory demonstrated this antibody works in Immunoprecipitation.
Independent laboratory demonstrated this antibody worked in Immunofluorescence studies at a 1:100-1:500 dilution.
Immunoprecipitation (IP):
Independent laboratory demonstrated this antibody works in Immunoprecipitation.
Quality
Western Blot Analysis:
This lot detected Rab13 at 1:1,000 (1g/mL) dilution in lysates from PC12 lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
This lot detected Rab13 at 1:1,000 (1g/mL) dilution in lysates from PC12 lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
Target description
25 kDa
Physical form
Affinity Purified rabbit IgG in storage buffer containing tri-citrate-phosphate, pH 7-8 and 0.1% sodium azide.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Katja Köhler et al.
The Journal of cell biology, 165(2), 175-180 (2004-04-21)
The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs) through a yet unknown mechanism. Here, we show that expression of the GTP-bound form of Rab13 inhibits PKA-dependent phosphorylation and TJ recruitment of the vasodilator-stimulated phosphoprotein, an actin
I Kanda et al.
Oncogene, 27(12), 1687-1695 (2007-09-25)
Epithelial cell scattering recapitulates the first steps of carcinoma invasion/metastasis. While the balance between cell-cell adhesive activity and cell motility ultimately determines this process, its molecular mechanisms remain unclear. Adherence junctions and tight junctions (TJs) are primarily responsible for cell-cell
Shinya Morimoto et al.
The Journal of biological chemistry, 280(3), 2220-2228 (2004-11-06)
During epithelial morphogenesis, adherens junctions (AJs) and tight junctions (TJs) undergo dynamic reorganization, whereas epithelial polarity is transiently lost and reestablished. Although ARF6-mediated endocytic recycling of E-cadherin has been characterized and implicated in the rapid remodeling of AJs, the molecular
Simone Di Giovanni et al.
The Journal of biological chemistry, 280(3), 2084-2091 (2004-11-04)
Following spinal cord injury, there are numerous changes in gene expression that appear to contribute to either neurodegeneration or reparative processes. We utilized high density oligonucleotide microarrays to examine temporal gene profile changes after spinal cord injury in rats with
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