05-257
Anti-p300 CT Antibody, clone RW128
clone RW128, Upstate®, from mouse
Synonym(s):
KAT3B
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RW128, monoclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... EP300(2033)
General description
p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
p300 and CBP are thought to increase gene expression in three ways:
(1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
(2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
(3) by acting as adaptor molecules.
p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
p300 and CBP are thought to increase gene expression in three ways:
(1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
(2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
(3) by acting as adaptor molecules.
Specificity
Recognizes p300 and co-immunoprecipitates E1A.
Immunogen
Epitope: C-terminus
GST-fusion to the C-Terminus of human p300
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
RNA Binding Protein (RBP)
Histones
Chromatin Biology
RNA Binding Protein (RBP)
Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.
Quality
routinely evaluated by immunoblot on HeLa nuclear extract
Target description
300 kDa
Physical form
Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol
Protein G Purified
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
E1A interacts with two opposing transcriptional pathways to induce quiescent cells into S phase.
Sha J, Ghosh MK, Zhang K, Harter ML
Journal of virology null
MAPK p38 regulates transcriptional activity of NF-kappaB in primary human astrocytes via acetylation of p65.
Saha, RN; Jana, M; Pahan, K
Journal of immunology (Baltimore, Md. : 1950) (1950)
Surabhi Dangi-Garimella et al.
PloS one, 8(5), e64566-e64566 (2013-05-23)
Pancreatic ductal adenocarcinoma (PDAC) is associated with a pronounced collagen-rich stromal reaction that has been shown to contribute to chemo-resistance. We have previously shown that PDAC cells are resistant to gemcitabine chemotherapy in the collagen microenvironment because of increased expression
Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy.
Franklin, S; Zhang, MJ; Chen, H; Paulsson, AK; Mitchell-Jordan, SA; Li, Y; Ping, P; Vondriska, TM
Molecular and Cellular Proteomics null
Qi-En Wang et al.
Nucleic acids research, 41(3), 1722-1733 (2013-01-01)
Besides the primary histone acetyltransferase (HAT)-mediated chromatin remodeling function, co-transcriptional factor, p300, is also known to play a distinct role in DNA repair. However, the exact mechanism of p300 function in DNA repair has remained unclear and difficult to discern
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