Direkt zum Inhalt
Merck
  • Mathematical modeling and experimental validation of the spatial distribution of boron in the root of Arabidopsis thaliana identify high boron accumulation in the tip and predict a distinct root tip uptake function.

Mathematical modeling and experimental validation of the spatial distribution of boron in the root of Arabidopsis thaliana identify high boron accumulation in the tip and predict a distinct root tip uptake function.

Plant & cell physiology (2015-02-12)
Akie Shimotohno, Naoyuki Sotta, Takafumi Sato, Micol De Ruvo, Athanasius F M Marée, Verônica A Grieneisen, Toru Fujiwara
ZUSAMMENFASSUNG

Boron, an essential micronutrient, is transported in roots of Arabidopsis thaliana mainly by two different types of transporters, BORs and NIPs (nodulin26-like intrinsic proteins). Both are plasma membrane localized, but have distinct transport properties and patterns of cell type-specific accumulation with different polar localizations, which are likely to affect boron distribution. Here, we used mathematical modeling and an experimental determination to address boron distributions in the root. A computational model of the root is created at the cellular level, describing the boron transporters as observed experimentally. Boron is allowed to diffuse into roots, in cells and cell walls, and to be transported over plasma membranes, reflecting the properties of the different transporters. The model predicts that a region around the quiescent center has a higher concentration of soluble boron than other portions. To evaluate this prediction experimentally, we determined the boron distribution in roots using laser ablation-inductivity coupled plasma-mass spectrometry. The analysis indicated that the boron concentration is highest near the tip and is lower in the more proximal region of the meristem zone, similar to the pattern of soluble boron distribution predicted by the model. Our model also predicts that upward boron flux does not continuously increase from the root tip toward the mature region, indicating that boron taken up in the root tip is not efficiently transported to shoots. This suggests that root tip-absorbed boron is probably used for local root growth, and that instead it is the more mature root regions which have a greater role in transporting boron toward the shoots.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Saccharose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Saccharose, ≥99.5% (GC)
Sigma-Aldrich
Saccharose, ≥99.5% (GC), BioXtra
Sigma-Aldrich
Saccharose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sigma-Aldrich
Borsäure, BioReagent, for molecular biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
Saccharose, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Saccharose, ≥99.5% (GC)
Sigma-Aldrich
Saccharose, ACS reagent
Sigma-Aldrich
Saccharose, ≥99.5% (GC), Grade II, suitable for plant cell culture
Sigma-Aldrich
Saccharose, Grade I, ≥99% (GC), suitable for plant cell culture
Sigma-Aldrich
Borsäure, suitable for electrophoresis, ≥99.5%
Sigma-Aldrich
Saccharose, puriss., meets analytical specification of Ph. Eur., BP, NF
Sigma-Aldrich
Saccharose, meets USP testing specifications
Sigma-Aldrich
Borsäure, 99.999% trace metals basis
Sigma-Aldrich
Borsäure, BioUltra, for molecular biology, ≥99.5% (T)