G005907

HPLC Analysis of Aflatoxins in Wheat on Ascentis^® Express C18 after SPE using Supel^™ Tox AflaZea

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample/matrix: 25 g wheat meal spiked with pesticide standards; SPE tube/cartridge: Supel^™Tox AflaZea, 6 mL (55314-U); sample addition: 2 mL; elution: collect eluate in 5 mL silanized culture tubes; eluate post-treatment: combine 200 μL of sample eluate and 880 μL of DI water in a 2 mL autosampler vial; column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U); mobile phase: [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid; flow rate: 0.400 mL/min; pressure: 4568 psi (315 bar); column temp.: 35 °C; detector: FLD 360 ex; 440 em; injection: 20 μL; derivatization: via KOBRA electrochemical cell, 50 μA
• Eignung: application for HPLC
• Anwendung(en): food and beverages

Beschreibung

Hinweis zur Analyse

Sample/matrix:
Weigh 25 grams of wheat meal into a 500 mL Erlenmeyer flask. Add 100 mL of 84:16 acetonitrile:water, seal, and place on an orbital shaker at med-high speed for 90 minutes. Filter the sample through a #2 qualitative filter. Spike sample at 2 mg/mL using analytical standards.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

Analyt

Aflatoxin G₂
Aflatoxin G₁
Aflatoxin B₂
Aflatoxin B₁