G005709

LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis^® Express RP Amide

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U); mobile phase: [A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B); flow rate: 0.5 mL/min; column temp.: 35 °C; detector: ESI(+), m/z 100-1000; injection: 0.5 μL; sample: 200 μg/L in 25:75, water: acetonitrile
• Eignung: application for HPLC
• Anwendung(en): forensics and toxicology

Beschreibung

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

EDDP perchlorate solution, 1.0 mg/mL in methanol (as pyrolinium), ampule of 1 mL, certified reference material, Cerilliant^®
(±)-Methadone solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
EMDP hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®