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Merck

50759-U

Supelco

Ascentis® Express RP-Amide, 5 μm HPLC Column

5 μm particle size, L × I.D. 25 cm × 3 mm

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About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

Materialien

stainless steel column

Qualitätsniveau

Agentur

suitable for USP L60

Produktlinie

Ascentis®

Leistungsmerkmale

endcapped

Hersteller/Markenname

Ascentis®

Verpackung

1 ea of

Parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

Methode(n)

HPLC: suitable
LC/MS: suitable

L × ID

25 cm × 3 mm

Oberflächenbereich

90 m2/g

Verunreinigungen

<5 ppm metals

Matrix

Fused-Core® particle platform
superficially porous particle

Aktive Matrixgruppe

amide, alkyl phase

Partikelgröße

5 μm

Porengröße

90 Å pore size

pH-Bereich

2-9

Anwendung(en)

food and beverages

Trenntechnik

reversed phase

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Allgemeine Beschreibung

Ascentis Express 5 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 μm thick porous shell and the highly uniform overall particle size of 5 μm. The one-step bonded, endcapped, amide-based, polar-embedded stationary phase of Ascentis Express 5 μm RP-Amide provides a stable, reversed-phase packing with decreased hydrophobic character. Ascentis Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from “phase collapse”.

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Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid
Fan Sai et al.
Journal of agricultural and food chemistry, 60(8), 1898-1905 (2012-02-15)
A sensitive method has been developed for the simultaneous determination of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLC-LIT-MS). This method is based on a new
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies

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