ZRB2827

Anti-FRS2/SNT-1 Antibody, clone 1C19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): FGFR substrate 2, FGFR-signaling adaptor SNT, Fibroblast growth factor receptor substrate 2, Suc1-associated neurotrophic factor target 1 (SNT-1)
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1C19, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 57.03 kDa; observed mol wt ~65 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Methode(n): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgGκ
• Epitopsequenz: Internal
• Protein-ID-Hinterlegungsnummer: NP_001265285
• UniProt-Hinterlegungsnummer: Q8WU20
• Grünere Alternativprodukt-Kategorie: Aligned
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... FRS2(10818)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1C19 is a ZooMAb^® recombinant rabbit monoclonal antibody that detects FRS2/SNT-1. It targets an epitope within 24 amino acids from internal region of the protein.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human FRS2/SNT-1.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected FRS2/SNT-1 in SH-SY5Y cell lysate.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected FRS2/SNT-1 in one million SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound FRS2/SNT-1 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FRS2/SNT-1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FRS2/SNT-1 in A-431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Fibroblast growth factor receptor substrate 2 (UniProt: Q8WU20; also known as FGFR substrate 2, FGFR-signaling adaptor SNT, Suc1-associated neurotrophic factor target 1 (SNT-1)) is encoded by the FRS2 (also known as ) gene (Gene ID: 10818) in human. Fibroblast Growth Factor Receptor Substrate 2 (FRS2) is an adaptor protein characterized by a myristoylated N-terminal region, which facilitates its membrane localization, and a phosphotyrosine-binding (PTB) domain that anchors it to the fibroblast growth factor receptor (FGFR). The C-terminal region of FRS2 contains multiple tyrosine residues that can be phosphorylated, forming docking sites for downstream signaling molecules such as Grb2 and SHP2. FRS2 plays a crucial role in mediating signal transduction from the FGFR to downstream pathways, primarily the MAPK/ERK and PI3K/AKT pathways, which are essential for cellular differentiation, growth, and survival. Upon FGFR activation, FRS2 becomes phosphorylated, serving as a scaffold to recruit and activate various signaling complexes. This activation is pivotal in translating extracellular growth factor signals into cellular responses. Overexpression or hyperactivation of FRS2 can lead to enhanced signaling through pathways that drive tumorigenesis.

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable