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YEAST1

Sigma-Aldrich

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Synonym(e):

lithium acetate yeast transformation

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.85

Qualität

for molecular biology

Qualitätsniveau

200

Verwendung

 kit sufficient for >100 standard transformations

Methode(n)

transformation: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Anwendung

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Leistungsmerkmale und Vorteile

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

Komponenten

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Prinzip

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1SDB
  • Y1501Yeast Synthetic Drop-out Medium Supplements, without uracil 1 gSDB

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Piktogramme

Health hazard
GHS08

Signalwort

Warning

H-Sätze

H373

Gefahreneinstufungen

STOT RE 2 Inhalation

Zielorgane

Respiratory Tract

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Mechanistic and structural insights into the regioselectivity of an acyl-CoA fatty acid desaturase via directed molecular evolution.
Vanhercke T
The Journal of Biological Chemistry, 286(15), 12860-12869 (2011)
Widening the pH activity profile of a fungal laccase by directed evolution.
Torres-Salas P
Chembiochem, 14(8), 934-937 (2013)
A simple and efficient procedure for transformation of yeasts.
R Elble
BioTechniques, 13(1), 18-20 (1992-07-01)
Alle zugehörigen Dokumente anzeigen

Artikel

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protokolle

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

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