Y3627

Hefe-Kohle-Basis

suitable for microbiology, NutriSelect^® Basic

• UNSPSC-Code: 41171606
• NACRES: NA.85

Eigenschaften

• Sterilität: non-sterile
• Qualitätsniveau: 200
• Form: powder
• Hersteller/Markenname: NutriSelect^® Basic
• Methode(n): microbe id | utilization test: suitable; microbiological culture: suitable
• Anwendung(en): clinical testing; food and beverages; life science and biopharma; microbiology
• Lagertemp.: 2-8°C
• Eignung: nonselective for Candida spp.; nonselective for Pichia spp.; nonselective for Saccharomyces spp.; nonselective for Zygosaccharomyces spp.

Beschreibung

Anwendung

Empfohlen für die Anwendung bei der Klassifizierung von Hefen anhand ihrer Fähigkeit, Stickstoff-Komponenten zu assimilieren.

Komponenten

Zusammensetzung (g/L)
Dextrose, 10.00
L-Histidin-hydrochlorid, 0.001
DL-Methionin, 0.002
DL-Tryptophan, 0.002
Biotin, 0.000002
Calciumpantothenat, 0.0004
Folsäure, 0.000002
Inositol, 0.002
Niacin, 0.0004
p-Aminobenzoesäure, 0.0002
Pyridoxin-hydrochlorid, 0.0004
Riboflavin (Vitamin B₂), 0.0002
Thiamin-hydrochlorid, 0.0004
Borsäure, 0.0005
Kupfersulfate, 0.00004
Kaliumiodid, 0.0001
Eisen(III)-chlorid, 0.0002
Mangansulfat, 0.0004
Natriummolybdat, 0.0002
Zinksulfat, 0.0004
mono-Kaliumphosphat, 1.00
Magnesiumsulfat, 0.50
Natriumchlorid, 0.10
Calciumchlorid, 0.10

Angaben zur Herstellung

A. Nitrogen Assimilation test
Prepare the broth base at 10 x concentration by dissolving 11.7 grams of Yeast Carbon Base in 100 mL of distilled water. Add the appropriate nitrogen source. Warm if necessary to dissolve the medium completely. Sterilize by filtration.
B. Detection of yeasts, other than Saccharomyces cerevisae
Dissolve 2.35 grams of Yeast Carbon Base in 100 mL of distilled water.
C. Detection of wild yeasts in beer and other brewing components
Add 0.33 g of ammonium sulphate (Catalog No. A2939) and 4 g of bacteriological agar (Catalog No. A5306) to base B. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Fußnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Gloves