U4002

Urease aus Canavalia ensiformis (Jack bean)

Type IX, powder, 50,000-100,000 units/g solid

• Synonym(e): Jack bean urease, Urea amidohydrolase
• CAS-Nummer: 9002-13-5
• EC-Nummer: 3.5.1.5 (BRENDA, IUBMB)
• EG-Nummer: 232-656-0
• MDL-Nummer: MFCD00070858
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Eigenschaften

• Typ: Type IX
• Form: powder
• Spezifische Aktivität: 50,000-100,000 units/g solid
• Mol-Gew.: ~544620 Da
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Molekulargewicht der Untereinheit: ~90,770
Besteht aus sechs Untereinheiten mit einem Molekulargewicht von ~544,620

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.

Anwendung

Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.

Biochem./physiol. Wirkung

In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.

Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.

Einheitendefinition

One micromolar unit will liberate 1.0 μmole of NH₃ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1