TP0200
Gesamtprotein-Kit, Micro-Lowry, Onishi & Barr Modifizierung
sufficient for ~50 manual assays
Synonym(e):
Total Protein Assay Kit
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352202
NACRES:
NA.32
Empfohlene Produkte
Beschreibung
wavelength 500-750nm
Verwendung
sufficient for ~50 manual assays
Messbereich Proteinnachweisgrenze
0-1 mg/mL
Parameter
18-26 °C temperature
200 μL sample volume
Lagertemp.
2-8°C
Allgemeine Beschreibung
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Anwendung
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Prinzip
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLSDB
- Folin & Ciocalteu’s phenol reagent 5 mL
Erforderlich, aber nicht bereitgestellt
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Lagerklassenschlüssel
8A - Combustible corrosive hazardous materials
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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