T8159
Tryptose-Phosphat-Bouillon -Lösung
sterile-filtered, suitable for cell culture
Synonym(e):
TPB solution
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About This Item
UNSPSC-Code:
12352205
NACRES:
NA.75
Empfohlene Produkte
Sterilität
sterile-filtered
Qualitätsniveau
Form
solution
Konzentration
29.5 g/L in deionized water
Methode(n)
cell culture | mammalian: suitable
Versandbedingung
ambient
Lagertemp.
room temp
Verwandte Kategorien
Anwendung
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Komponenten
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
Vergleichbares Produkt
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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