T6455
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever
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About This Item
UNSPSC-Code:
12352107
NACRES:
NA.47
Empfohlene Produkte
Biologische Quelle
synthetic
Qualitätsniveau
pH-Wert
10
Anwendung(en)
hematology
histology
Lagertemp.
2-8°C
Anwendung
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Angaben zur Herstellung
Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.
Lagerung und Haltbarkeit
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
Sonstige Hinweise
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Sens. 1
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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