SRP6119

DHFR from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(e): DYR, Dihydrofolate reductase, N/A
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Mol-Gew.: 23.8 kDa (207 aa, 1-187 aa + NT His-Tag)
• Verpackung: pkg of 100 μg
• NCBI-Hinterlegungsnummer: P00375
• UniProt-Hinterlegungsnummer: P00375
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: mouse ... DHFR(13361)

Beschreibung

Allgemeine Beschreibung

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. The gene encoding this protein is localized on mouse chromosome 13.

Biochem./physiol. Wirkung

DHFR catalyzes the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Physikalische Form

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.

Angaben zur Herstellung

Centrifuge the vial prior to opening.

Sonstige Hinweise

MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable