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Merck

SRP3211

Sigma-Aldrich

IL-4 from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(e):

B cell stimulating factor (BSF-1), BCDF, BCGF

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

mouse

Rekombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

Form

lyophilized

Wirksamkeit

≤2.0 ng/mL ED50

Mol-Gew.

13.7 kDa

Verpackung

pkg of 20 μg

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

<0.1 EU/μg endotoxin, tested

Farbe

white to off-white

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

mouse ... IL4(16189)

Allgemeine Beschreibung

IL-4 (interleukin 4) is a pleiotropic cytokine. Produced by mast cells, T cells and bone marrow stromal cells. Recombinant murine IL-4 is a 13.7kDa globular protein containing 120 amino acid residues.

Biochem./physiol. Wirkung

It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.

Sequenz

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Physikalische Form

Lyophilized with no additives.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Chih-Wei Ko et al.
Bio-protocol, 8(17), e3003-e3003 (2018-09-05)
Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently related and unrelated pathways of energy metabolism.
Mast cell production and response to IL-4 and IL-13.
McLeod JJ
Cytokine, 75, 57-61 (2015)
Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.
Hasbold J
European Journal of Immunology, 28, 1040-1051 (1998)
Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells.
Pawankar R
The Journal of Clinical Investigation, 99, 1492-1492 (1997)
IL-4 Knock Out Mice Display Anxiety-Like Behavior.
Moon ML
Behavior Genetics, 45, 451-460 (2015)

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