SRP3013

Beta D-4 human

recombinant, expressed in E. coli, ≥98% (HPLC), suitable for cell culture

• Synonym(e): Beta-defensin 104, DEFB104A
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC)
• Form: lyophilized
• Mol-Gew.: 6.0 kDa
• Verpackung: pkg of 20 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white to off-white
• Eignung: suitable for
• UniProt-Hinterlegungsnummer: Q8WTQ1
• Wirkungsspektrum von Antibiotika: fungi; mycobacteria; viruses
• Wirkungsweise: cell membrane | interferes
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... DEFB104A(140596)

Beschreibung

Allgemeine Beschreibung

Chemical structure: peptide

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human b-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Biochem./physiol. Wirkung

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Sequenz

EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Physikalische Form

Lyophilized from 10 mM Acetic Acid.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H315,H319
• P-Sätze: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2 - Skin Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable