Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

SRP2120

Sigma-Aldrich

RNA Polymerase II Peptide

≥70% (SDS-PAGE), human recombinant, expressed in E. coli, GST tagged

Synonym(e):

POLRA, RPB1, RPO2, RPOL2, hRPB220

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12352202
NACRES:
NA.26

product name

RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Biologische Quelle

human

Rekombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

Form

frozen liquid

Mol-Gew.

~68.1 kDa

Verpackung

pkg of 10 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Farbe

clear colorless

NCBI-Hinterlegungsnummer

NM_000937

UniProt-Hinterlegungsnummer

P24928

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... POLR2A(5430)

Verwandte Kategorien

Biochem./physiol. Wirkung

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo: IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongation complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
The CTD works as a binding scaffold for nuclear factors through its phosphorylation. It is suggested to be also involved in chromatin structure modification, DNA damage/repair, protein degradation and synthesis, RNA degradation, snRNA (small nuclear RNA) modification, and snoRNP (small nucleolar ribonucleoprotein) biogenesis.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

RNA polymerase II C-terminal domain required for enhancer-driven transcription.
Gerber HP, et al.
Nature, 374, 660-662 (1995)
Phosphorylation and functions of the RNA polymerase II CTD.
Phatnani HP and Greenleaf AL
Genes & Development, 20, 2922-2936 (2006)
T O'Brien et al.
Nature, 370(6484), 75-77 (1994-07-07)
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II is essential in vivo, and is found in either an unphosphorylated (IIa) or hyperphosphorylated (IIo) form. The Drosophila uninduced hsp70 and hsp26 genes, and the constitutively expressed beta-1
H Lu et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(22), 10004-10008 (1991-11-15)
The two forms of RNA polymerase II that exist in vivo, phosphorylated (IIO) and nonphosphorylated (IIA), were purified to apparent homogeneity from HeLa cells. The nonphosphorylated form preferentially binds to the preinitiation complex. RNA polymerase II in the complex was
The role of multisite phosphorylation in the regulation of RNA polymerase II activity.
M E Dahmus
Progress in nucleic acid research and molecular biology, 48, 143-179 (1994-01-01)
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.