SRP0734

PIP4K2B, Active

PRECISIO^® Kinase, recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.27

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: 4500-6000 nmol/min·mg (ADP-Glo)
• Mol-Gew.: ~73 kDa
• Konzentration: 0.1 μg/μL
• Hinterlegungsnummer: NM_003559
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PIP4K2B(8396)

Beschreibung

Anwendung

Useful for kinase assay.

Biochem./physiol. Wirkung

Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha ⁽¹⁾. PIP5P4KB plays a role in determining insulin sensitivity and adiposity ⁽²⁾.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable