SRP0690

LKB1(STK11) Protein

recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• Form: frozen liquid
• Mol-Gew.: ~85 kDa
• Konzentration: 0.2 μg/μL
• Hinterlegungsnummer: NM_000455
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... LKB1(6794)

Beschreibung

Anwendung

Useful for Western blot and in kinases assays as a downstream substrate for SRC or MEK1 kinases.

Biochem./physiol. Wirkung

Recombinant full-length human LKB1(STK11) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. LKB1(STK11) is a member of the serine/threonine kinase family which regulates cell polarity and functions as a tumor suppressor. STK11 is a critical barrier to pulmonary tumorigenesis which controlling initiation, differentiation, and metastasis. STK11 plays a central role in restricting HSC entry into cell cycle and in broadly maintaining energy homeostasis in hematopoietic cells through a novel metabolic checkpoint ⁽¹⁾. STK11 serves as an essential regulator of HSCs and hematopoiesis, and more generally, points to the critical importance of coupling energy metabolism and stem cell homeostasis ⁽²⁾.

Rekonstituierung

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Lagerung und Haltbarkeit

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable