SRP0339

Ceramide Kinase FLAG tag active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)

• Synonym(e): CERK, LK4, hCERK
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥40% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 60 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 0.4 mg/mL
• NCBI-Hinterlegungsnummer: NM_022766
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CERK(64781)

Beschreibung

Allgemeine Beschreibung

Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.

Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.

Einheitendefinition

One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable