SRP0217

SHP-2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(e): Noonan syndrome 1, PTP-1D, PTP2C, PTPN11, SHP2
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 59.5 kDa
• Verpackung: pkg of 20 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: 0.73 mg/mL
• NCBI-Hinterlegungsnummer: NM_002834
• UniProt-Hinterlegungsnummer: Q06124
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PTPN11(5781)

Beschreibung

Allgemeine Beschreibung

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.

Anwendung

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.

Biochem./physiol. Wirkung

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.

Einheitendefinition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sonstige Hinweise

Amino acids : 224-529

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable