SAE0198

Calprotectin, human recombinant

Stabilized heterodimer of fusion tagged S100A9 and S100A8

• Synonym(e): MRP8/14 and S100A8/A9 complex
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Qualitätsniveau: 200
• Assay: ≥90% (SDS-PAGE)
• Form: lyophilized powder
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis-SUMO tag is added to the N-terminus of S100A9, and a 6xHIS tag is added to the N-terminus of S100A8. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).

Anwendung

Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease¹. This product is a recombinant version of the human protein found endogenously.

Biochem./physiol. Wirkung

Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.¹ The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,² and its antimicrobial activity possibly through chelating of Zn.³ High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.⁴ A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.⁵

Physikalische Form

The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.

Angaben zur Herstellung

Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1