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SAB4200762

Sigma-Aldrich

Anti-Opsin antibody, Mouse monoclonal

clone RET-P1, purified from hybridoma cell culture

Synonym(e):

Anti-Opsin 2, Anti-Rhodopsin

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

200

Antikörperform

purified from hybridoma cell culture

Antikörper-Produkttyp

primary antibodies

Klon

RET-P1, monoclonal

Form

buffered aqueous solution

Mol-Gew.

~39 kDa

Speziesreaktivität

avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit

Konzentration

~1 mg/mL

Methode(n)

ELISA: suitable
immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract.
immunofluorescence: suitable
immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.
radioimmunoassay: suitable

Isotyp

IgG1

UniProt-Hinterlegungsnummer

P08100

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

rat ... Rho(24717)

Allgemeine Beschreibung

Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.

Anwendung

Anti-Opsin antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • radioimmuno assay (RIA)

Biochem./physiol. Wirkung

Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.

Sonstige Hinweise

rat retinal membranes

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

The opsins
Terakita A.
Genome Biology, 6(3), 213-213 (2005)
Retinitis pigmentosa
Hamel C.
Orphanet Journal of Rare Diseases, 1(1), 40-40 (2006)
Yuanyuan Chen et al.
The Journal of biological chemistry, 289(13), 9288-9303 (2014-02-12)
The P23H opsin mutation is the most common cause of autosomal dominant retinitis pigmentosa. Even though the pathobiology of the resulting retinal degeneration has been characterized in several animal models, its complex molecular mechanism is not well understood. Here, we
P A Hargrave et al.
Experimental eye research, 42(4), 363-373 (1986-04-01)
Antisera and monoclonal antibodies to rhodopsin were examined for their binding specificity to rhodopsin by using peptides from the rhodopsin sequence as competitors for antibody binding to rhodopsin in an enzyme-linked immunoassay. Monoclonal antibodies tested were raised in mice against
T Watanabe et al.
Development (Cambridge, England), 114(4), 899-906 (1992-04-01)
We previously developed a reaggregate cell culture system in which embryonic rat retinal neuroepithelial cells proliferate and give rise to opsin-expressing rod photoreceptor cells (rods) on the same schedule in vitro as they do in vivo. We showed that the
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