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Merck

SAB4200688

Sigma-Aldrich

Anti-Bovine Serum Albumin antibody, Mouse monoclonal

clone BSA-33, purified from hybridoma cell culture

Synonym(e):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

BSA-33, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~70 kDa

Speziesreaktivität

sheep (highly reactive ), horse (less reactive ), dog (less reactive ), turkey (less reactive ), goat (highly reactive )

Konzentration

~1 mg/mL

Methode(n)

immunoblotting: 0.5-1 μg/mL using Fetal Bovine Serum.
immunoprecipitation (IP): 1-2 μg using Fetal Bovine Serum.
indirect ELISA: 0.03-0.075 μg/mL using 5 μg/mL BSA for coating.

Isotyp

IgG2a

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

dog ... Alb(403550)
horse ... Alb(100034206)
sheep ... Alb(443393)

Allgemeine Beschreibung

Serum albumin, the most abundant protein (30–50 mg/mL) in the plasma, is a multifunctional, non-glycosylated, negatively charged protein produced in the liver. It binds to a wide variety of lipophilic compounds such as metal ions, pharmaceuticals and metabolites. Albumin regulates osmotic pressure in the blood and has a role in the transport of fatty acids.

Immunogen

Bovine Serum Albumin (BSA)

Physikalische Form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Sonstige Hinweise

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Albumin: Biochemical properties and therapeutic potential*
Gregory J. Quinlan, et al.
Hepatology (2005)
Clinical use of albumin in hepatology
Matteo G, et al.
Blood Transfusion = Trasfusione del Sangue, 7(4), 268-277 (2009)
Baker, M.E.,
The Journal of Endocrinology, 175, 121-17 (2002)
Poonam Rajak et al.
Journal of pharmaceutical and biomedical analysis, 78-79, 154-160 (2013-03-19)
Proteins present in human serum are of immense importance in the field of biomarker discovery. But, the presence of high-abundant proteins like albumin makes the analysis more challenging because of masking effect on low-abundant proteins. Therefore, removal of albumin using

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