SAB4200053

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

• Synonym(e): Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: PDG 5G1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~125 kDa
• Speziesreaktivität: human, mouse, rat
• Verpackung: antibody small pack of 25 μL
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using Jurkat cells extract
• UniProt-Hinterlegungsnummer: P28340
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... POLD1(5424)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

Immunogen

human p125

Anwendung

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• co-immunoprecipitation

Biochem./physiol. Wirkung

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Physikalische Form

Lösung in 0.01 M Phosphat-gepufferter Kochsalzlösung pH 7.4, enthält 15 mM Natriumazid.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable