S9571

Anti-SUMO 2 in Kaninchen hergestellte Antikörper

~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-SMT3B, Anti-SMT3H2, Anti-Sentrin-2
• MDL-Nummer: MFCD03455941
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~15 kDa
• Speziesreaktivität: human
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~0.6 mg/mL
• Methode(n): indirect immunofluorescence: 10-20 μg/mL using HeLa cells; western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells or cell extract of HEK293-T cells transfected with human SUMO-2
• UniProt-Hinterlegungsnummer: P61956
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SUMO2(6613) ; mouse ... Sumo2(170930)

Beschreibung

Allgemeine Beschreibung

Small ubiquitin-related modifier (SUMO-2) gene is mapped to human chromosome 17q25.1. Sumo-2 is related to SUMO-3 with a 86% sequence identity. It comprises a sumoylation motif at the residue lysine 11.

Spezifität

Anti-SUMO-2 recognizes human SUMO-2.

Immunogen

synthetic peptide corresponding to amino acids 80-93 located at the C-terminal of human SUMO-2, conjugated to KLH. This sequence is identical in many species including rat, mouse, pig, and bovine SUMO-2 and is identical in human SUMO-3.

Anwendung

Anti-SUMO-2 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem./physiol. Wirkung

Small ubiquitin-related modifier (SUMO-2) is essential for the cellular defense in hyperthermic cytotoxicity. Along with SUMO-3, SUMO2 is essential for cell survival post-heat shock. SUMO-2 has the ability to exist as self-modified polymers. SUMO-2 and SUMO-3 negatively regulate interferon induction.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% bovien serum albumin and 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)