S6436

Anti-phospho-S6-Kinase (pThr⁴²¹/pSer⁴²⁴) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(e): Anti-phospho-p70^S6K (pThr⁴²¹/pSer⁴²⁴)
• MDL-Nummer: MFCD01092451
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous glycerol solution
• Speziesreaktivität: mouse, rat, human
• Methode(n): immunoprecipitation (IP): 1:250; western blot (chemiluminescent): 1:1,000
• UniProt-Hinterlegungsnummer: P23443
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... RPS6KB1(6198) ; mouse ... Rps6kb1(72508) ; rat ... Rps6kb1(83840)

Beschreibung

Allgemeine Beschreibung

S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.

Spezifität

Does not react with non-phosphorylated S6 kinase (p70^S6K) or other phosphorylated protein kinases.

Immunogen

synthetic double phospho-Thr⁴²¹/Ser⁴²⁴ peptide corresponding to residues around Thr⁴²¹/Ser⁴²⁴ of human p70^S6K.

Anwendung

Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.

Physikalische Form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable