RAB0510

Mouse VEGF ELISA Kit

for cell and tissue lysates

• Synonym(e): VEGF-A
• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: mouse
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable; capture ELISA: suitable
• Aufnahme: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2 pg/mL; standard curve range: 4.1-1000 pg/mL
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Vegfa(22339)

Beschreibung

Allgemeine Beschreibung

The Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF cell lysate and tissue lysate.

Immunogen

Recombinant Mouse VEGF-A

Anwendung

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse VEGF ELISA Kit has been used to determine the concentration of VEGF expression in samples using ELISA.

Biochem./physiol. Wirkung

Vascular endothelial growth factor (VEGF) is a critical factor in regulating angiogenesis in both physiological and pathological conditions. Transforming growth factor-α (TGF-α), TGF-β and platelet derived growth factor can potentially activate VEGF activity. VEGF mediates its activity through tyrosine kinase receptors, vascular endothelial growth factor receptor-1 (VEGFR-1) and VEGFR-2. VEGF produced by tumour cells and macrophages, plays a key role in cancer development by promoting endothelial cells proliferation and increasing vascular permeability. Enhanced expression of VEGF plays a key role in urologic neoplasm like bladder cancer, renal cell carcinoma, and cervical cancer. Polymorphism in VEGF gene increases the disease susceptibility. Blocking of VEGF mediated signalling pathway is a potential therapeutic strategy for cancer patients.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8B - Non-combustible corrosive hazardous materials