R8529

Monoclonal Anti-RAL-A antibody produced in mouse

~2 mg/mL, clone RalA5F9.2, purified immunoglobulin, buffered aqueous solution

• MDL-Nummer: MFCD08705597
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: RalA5F9.2, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 25 kDa
• Speziesreaktivität: monkey, mouse, human, rat
• Konzentration: ~2 mg/mL
• Methode(n): immunocytochemistry: 5-10 μg/mL using HeLa cells; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using rat brain extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P11233
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RALA(5898) ; mouse ... Rala(56044) ; rat ... Rala(81757)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.

Spezifität

Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.

Immunogen

synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.

Anwendung

Monoclonal Anti-RAL-A antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)

Biochem./physiol. Wirkung

Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For extended storage, freeze at –20 °C in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)