R1008

Monoklonaler Anti-Kaninchen-IgG-Antikörper (γ-kettenspezifisch)

clone RG-96, ascites fluid

• Synonym(e): Monoclonal Anti-Rabbit IgG (γ-chain specific)
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: secondary antibodies
• Klon: RG-96, monoclonal
• Enthält: 15 mM sodium azide
• Speziesreaktivität: rabbit
• Darf nicht reagieren mit: goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
• Methode(n): dot blot: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: 1:2,000; western blot: suitable
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Das Produkt erkennt ein Epitop, das sich an der γ-(Schwer-)Kette von Kaninchen-IgG befindet. Beim Immunblotting erkennt der Antikörper sowohl native als auch denaturierte Formen von Kaninchen-IgG. In ELISA ist der Antikörper spezifisch für Kaninchen-IgG und zeigt keine Kreuzreaktivität mit Kaninchen-IgA und -IgM oder Human-IgG, -IgA und -IgM. Es wurde keine Kreuzreaktion mit IgG von folgenden Spezies festgestellt: Rind, Katze, Huhn, Hund, Ziege, Meerschweinchen, Pferd, Schwein, Ratte oder Schaf.

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Spezifität

Monoclonal Anti-Rabbit IgG is specific for an epitope located on the heavy (γ) chain of rabbit IgG, in an immunoblot of denatured, reduced rabbit IgG. The antibody does not react with rabbit IgA, IgM or light chains. No cross reactivity is observed with IgG from bovine, cat, chicken, dog, goat, guinea pig, horse, human, pig, rat or sheep.

Anwendung

Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse has been used in

• enzyme linked immunosorbent assay (ELISA)
• dot blots
• immunohistology
• western blotting
• peptide mass fingerprinting (PMF)

Biochem./physiol. Wirkung

Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc region is involved in the development of therapeutic antibody.

Physikalische Form

The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable