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ProteoPrep® Total Extraction Sample Kit
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About This Item
UNSPSC-Code:
12352200
NACRES:
NA.32
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Allgemeine Beschreibung
ProteoPrep® Total Extraction Sample Kit is designed to extract all proteins from any type of cell or tissue. It includes two reagents that have been used extensively for extraction and two new reagents with increased solubilizing power to extract more proteins. This kit also includes reagents for the reduction and alkylation of disulfide bonds. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels.
Anwendung
ProteoPrep® Total Extraction Sample Kit has been used to extract total protein from various sources such as epidermal stem cells and rheumatoid arthritis (RA) synovial fibroblasts.
Leistungsmerkmale und Vorteile
- Four pre-mixed solubilization reagents - Enables rapid solubilization.
- Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
- Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.
Lagerung und Haltbarkeit
These reagents should remain stable for at least 6 months in their unopened container.
Sonstige Hinweise
The ProteoPrep® Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
This kit contains enough reagents to generate at least ten 2-ml samples using all four reagents. For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.
Rechtliche Hinweise
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Samples containing high amount of salts and buffers may not work with this kit. These samples should be dialyzed prior to use. This product is for R&D use only, not for drug, household, or other uses.
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- C0481Protein Extraction Reagent Type 1SDB
- C0606Protein Extraction Reagent Type 2SDB
- C0731Protein Extraction Reagent Type 3SDB
- C0356Protein Extraction Reagent Type 4SDB
- A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDB
- T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDB
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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